PROXIMAL TUBULE (PTE) CELL CULTURE

• Isolation of human proximal tubule cells
• Preparation of matrix substrate
• Maintenance of cells in culture
• Subculture procedure
• Cryopreservation

PTEC CHARACTERISATION

• Light microscopy
• Electron microscopy
• Immunofluorescence
• Enzymatic activity
• Homornal stimulation for cAMP production
• Sodium dependent glucose uptake

RNA and MICROARRAY

• RNA isolation, quantitation and electrophoresis
• Microarray protocol